Plasmid

Part:BBa_K3779005

Designed by: JiaCheng, Li   Group: iGEM21_NWU-CHINA-B   (2021-06-19)


pPIC9K

Multiple plasmid integration events occur spontaneously in Pichia at a frequency between 1 and 10% of all His + transformants. The in vivo method allows you to screen for the His + transformants that may have multiple inserts of your gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1793
    Illegal NheI site found at 2740
    Illegal NotI site found at 1234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 6874
    Illegal BamHI site found at 938
    Illegal XhoI site found at 1192
    Illegal XhoI site found at 5709
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4080
    Illegal BsaI site found at 6181
    Illegal BsaI.rc site found at 8246
    Illegal SapI site found at 7163


[edit]
Categories
//awards/composite_part
Parameters
chassis